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Company: NEWA Tecno Industria
Catalog#: NJ600
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Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light
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2017-07-20
[Abstract]  Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes. [摘要]  对环境线索的根系反应动态进行表型分析是了解植物适应环境的必要条件。 持续监测根系生长是有挑战性的,因为根系通常生长在地下,对其生长环境非常敏感。 该方案将红外成像与水培培养相结合,用于运动学分析。 它允许在精细的时空分辨率下连续成像并最小化干扰根。 提供了如何将程序和材料适用于3D监测以及为实验目的如何操纵环境压力的实例。
【背景】运动学分析用于监测植物的生长和向性可以追溯到19世纪末期,早期研究从朱利叶斯·冯·萨克斯和惠尔普菲弗。 20世纪20年代以前摄影的广泛应用导致了通过“连拍摄影”(List of 1969; Erickson和Silk,1980)更容易和持续的监控。在90年代,新的数码相机和信息学工具使得能够开发用于粒子图像测速的自动跟踪算法。 RootFlowRT(Van der Weele等人,2003),Kineroot(Basu等人,2007),RootTrace(French et al。,et al。,& ...

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