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Zinc sulfate heptahydrate RPE - For analysis - ACS 1 kg

Company: CARLO ERBA Reagents
Catalog#: 494907
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Quantification of Chlorophyll as a Proxy for Biofilm Formation in the Cyanobacterium Synechococcus elongatus
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2017-07-20
[Abstract]  A self-suppression mechanism of biofilm development in the cyanobacterium Synechococcus elongatus PCC 7942 was recently reported. These studies required quantification of biofilms formed by mutants impaired in the biofilm-inhibitory process. Here we describe in detail the use of chlorophyll measurements as a proxy for biomass accumulation in sessile and planktonic cells of biofilm-forming strains. These measurements allow quantification of the total biomass as estimated by chlorophyll level and representation of the extent of biofilm formation by depicting the relative fraction of chlorophyll in planktonic cells. [摘要]  最近报道了蓝细菌聚球蓝细菌PCC 7942中生物膜发育的自我抑制机制。 这些研究需要定量由生物膜抑制过程中受损的突变体形成的生物膜。 在这里,我们详细描述了叶绿素测量作为生物膜形成菌株无菌和浮游细胞中生物量积累的代用途。 这些测量可以通过叶绿素水平估计的总生物量进行定量,并通过描绘浮游细胞中叶绿素的相对分数来表示生物膜形成的程度。
【背景】几个最近发表的研究表明,蓝藻中细胞聚集和生物膜发育的基础的机制的新兴兴趣(Fisher等人,2013; Jittawuttipoka等人,2013; 2014; Enomoto等人,2014; Schwarzkopf等人,2014; Enomoto等人,,2015; Oliveira等人,2015; Agostoni等人,2016; Parnasa等人,2016)。我们最近报道了一种自生物膜抑制机制,其决定了单细胞蓝细菌聚球蓝细菌PCC 7942(Schatz等人,2013; Nagar和Schwarz,2015)的浮游生长)。通过灭活特定基因来消除生物膜抑制过程导致在这种其他浮游菌株(Schatz等人,2013; ...

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