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Bromophenol Blue sodium salt


Company: Sigma-Aldrich
Catalog#: 114405
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Determination of VPS34/PIK3C3 Activity in vitro Utilising 32P-γATP
[Abstract]  VPS34 is the only class III phosphatidylinositol-3-kinase (PI3K) in mammalian cells and produces the vast majority of cellular phosphatidylinositol-3-phosphate [PI(3)P]. PI(3)P is a key signalling lipid that plays many membrane trafficking roles in processes such as endocytosis and autophagy. VPS34 is a key cellular regulator, loss of function can have catastrophic effects and is embryonic lethal (Zhou et al., 2011). The levels of cellular PI(3)P can be determined by fluorescent staining techniques and can be used to monitor effects upon VPS34 activity, however it is important to verify that any changes are mediated by VPS34, particularly as alternate pathways of PI(3)P production are possible such as via class II PI3Ks (Devereaux et al., 2013). Assaying VPS34 activity ... [摘要]  VPS34是哺乳动物细胞中唯一的III类磷脂酰肌醇-3-激酶(PI3K),并且产生绝大多数细胞磷脂酰肌醇-3-磷酸[PI(3)P]。 PI(3)P是一个关键的信号脂质,在诸如内吞作用和自噬的过程中起许多膜运输的作用。 VPS34是关键的细胞调节剂,功能丧失可具有灾难性作用并且是胚胎致死的(Zhou等人,2011)。 细胞PI(3)P的水平可以通过荧光染色技术确定,并且可以用于监测对VPS34活性的影响,然而重要的是验证任何变化由VPS34介导,特别是作为PI(3)的替代途径, P生产是可能的,例如通过II类PI3K(Devereaux等人,2013)。 直接在体外测定VPS34活性可以是描绘特定刺激的作用的关键阶段。