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Author:
Date:
2017-07-05
[Abstract] Coating tissue culture vessels with the components of the extracellular matrix such as fibronectin and collagens provides a more natural environment for primary cells in vitro and stimulates their proliferation. However, the effects of such protein layers are usually rather modest, which might be explained by the loss immobilized proteins due to their weak non-covalent association with the tissue culture plastic. Here we describe a simple protocol for a controlled fixation of fibronectin, vitronectin and collagen IV layers by formaldehyde, which substantially enhances the stimulation of primary cell proliferation by these extracellular proteins.
[摘要] 用诸如纤连蛋白和胶原的细胞外基质的组分涂覆组织培养容器为体外原代细胞提供更自然的环境并刺激它们的增殖。 然而，这种蛋白质层的作用通常相当适中，这可能由于与组织培养塑料的非共价结合弱而被固定蛋白质损失所解释。 在这里我们描述一个简单的协议，通过甲醛控制纤维连接蛋白，玻连蛋白和胶原IV层的固定，这大大增强了这些细胞外蛋白对原代细胞增殖的刺激。
【背景】细胞外基质（ECM）如纤连蛋白，层粘连蛋白，玻连蛋白和胶原蛋白的组分通常用于包被组织培养容器，因为它们为体外原代细胞提供更自然的环境并刺激它们的增殖（ Sawada等人，1987; ...
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