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Author:
Date:
2017-07-05
[Abstract] To investigate whether endothelial Akt1 activation is sufficient to induce vascular tumor formation in the skin, we have developed a skin graft model in which a skin fragment from transgenic donor mice with inducible and endothelial cell-specific overexpression of activated Akt1 (myrAkt1) is grafted into the skin of wild type recipient mice. The donor skin successfully engrafts after two weeks and, more importantly, vascular tumor develops at the site of transgenic skin graft when myrAkt1 expression is turned on. This skin graft model is a novel approach to investigate the biological impact of a key signal transduction molecule in a temporal, localized and organ-specific manner.
[摘要] 为了研究内皮Akt1的活化是否足以诱导皮肤血管肿瘤的形成，我们开发出一种皮肤移植模型，其中转基因供体小鼠的皮肤片段具有诱导型和内皮细胞特异性过度表达的活化的Akt1（myrAkt1）被移植入 野生型受体小鼠的皮肤。 供体皮肤在两周后成功植入，更重要的是，当myrAkt1表达被打开时，血管肿瘤发生在转基因皮肤移植物的位点。 这种皮肤移植模型是一种新颖的方法，以时间，局部和器官特异性方式研究关键信号转导分子的生物学影响。
【背景】我们的研究重点是调查Akt1在血管肿瘤发展中的作用。为了确定内皮细胞中Akt1的激活是否足以驱动血管瘤形成，我们开发并发表了一种血管肿瘤的皮肤移植模型（Phung et al。 ...
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