| LT-HSC Methylcellulose Assay
|
|
Author:
Date:
2014-03-05
[Abstract] Hematopoietic differentiation is a highly complex process originating from an extraordinary population of cells called long-term repopulating hematopoietic stem cells (LT-HSCs). The unique feature of all stem cells, including HSCs, is their exceptional ability to divide asymmetrically giving rise to two different kinds of offspring. One daughter cell becomes an LT-HSC itself (self-renews) to maintain the LT-HSC pool, whereas the second daughter cell pursues a differentiation fate to ultimately give rise to terminally differentiated mature blood cells (Orkin and Zon, 2008). Quantification of phenotypic LT-HSCs can be performed by multi-color flow cytometry and the gold standard for assessment of LT-HSC self-renewal and function is competitive bone marrow transplantation (Miller et al. ...
[摘要] 造血分化是一种非常复杂的过程，源自称为长期重建造血干细胞（LT-HSCs）的非凡细胞群。所有干细胞（包括HSC）的独特之处在于其不对称的划分能够产生两种不同种类的后代。一个子细胞成为LT-HSC本身（自我更新）以维持LT-HSC库，而第二个子细胞追求分化命运，最终产生终末分化的成熟血细胞（Orkin和Zon，2008）。表型LT-HSC的定量可以通过多色流式细胞术进行，用于评估LT-HSC自我更新和功能的黄金标准是竞争性骨髓移植（Miller等，2008）。尽管这些方法对于确定LT-HSC丰度和功能是不可替代的，但它们具有其缺点和局限性。例如，竞争性骨髓移植通常被监测为12-16周内外周血献血者贡献的函数。虽然减少的外周血供体贡献本身表示干/祖细胞隔室中的损伤，但是它不能明确区分降低的LT-HSC自我更新，受损的LT-HSC分化或受损的祖细胞分化。这里我们描述一种LT-HSCs甲基纤维素集落形成测定法，作为直接评估LT-HSC分化能力的快速互补体外方法。如Kerenyi等人所述（2013），该技术是区分LT-HSC或祖细胞分化缺陷的有力工具。
|
|
|
| IFN-α Inhibition Assay in vitro
|
|
Author:
Date:
2013-06-20
[Abstract] During viral infections Interferon-α (IFN-α) is expressed by infected host cells. IFN-α binds to its receptor (IFNAR1/2), which leads to the activation of downstream signaling via JAK-STAT. This signaling cascade results in the expression of several hundred different genes, so called interferon-stimulated gene, which lead to an antiviral state of the infected and the neighboring cells.
[摘要] 在病毒感染期间，干扰素-α（IFN-α）由感染的宿主细胞表达。 IFN-α结合其受体（IFNAR1/2），其导致通过JAK-STAT的下游信号传导的激活。 这种信号级联导致数百种不同基因的表达，所谓的干扰素刺激基因，其导致感染细胞和相邻细胞的抗病毒状态。
|
|
|
| Measurement of IFN-α Subtype Concentrations (Virus-free, Cell-based Bioassay)
|
|
Author:
Date:
2013-06-20
[Abstract] The induction of type I IFN is the immediate host response against viral infections. Type I IFNs belong to a multigene family including up to 14 different IFN-α subtypes and one IFN-β. They are highly conserved and bind the same receptor (IFNAR1/2) with varying affinities, although they differ in their biological activities.
[摘要] I型IFN的诱导是针对病毒感染的直接宿主应答。 I型IFNs属于多基因家族，包括多达14种不同的IFN-α亚型和一种IFN-β。 它们是高度保守的，并以不同的亲和力结合相同的受体（IFNAR1/2），尽管它们的生物活性不同。
|
|
|