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Author:
Date:
2016-08-20
[Abstract] 15N is a nonradioactive heavy isotope of nitrogen, widely used for biochemical and physiological research in plants. For instance, 15N-KNO3 was used as the nitrogen source in plants in order to investigate nitrate uptake activity and transport from roots to shoots (Lin et al., 2008). Here, we describe a detailed pipeline used for labeling living rice (Oryza sativa) plants with 15N-KNO3 and determination of net nitrate uptake and transport activity, and this protocol was proved to be valid in Arabidopsis and rice (Lin et al., 2008; Hu et al., 2015).
[摘要] N是氮的非放射性重同位素，广泛用于植物的生化和生理研究。 例如，使用N-KNO 3作为植物中的氮源，以研究硝酸盐吸收活性和从根到芽的转运（Lin等人 。， 2008）。 在这里，我们描述了用于标记具有 N-KNO 3的活水稻（>稻）植物和测定硝酸盐硝酸盐吸收的详细管道 和转运活性，并且该方案在拟南芥（Arabidopsis）和水稻中被证明是有效的（Lin等人，2008; Hu等人， 2015）。
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