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BenchMarkTM Fetal Bovine Serum

BenchMark TM胎牛血清

Company: Gemini Bio-Products
Catalog#: 100106
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LT-HSC Methylcellulose Assay
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2014-03-05
[Abstract]  Hematopoietic differentiation is a highly complex process originating from an extraordinary population of cells called long-term repopulating hematopoietic stem cells (LT-HSCs). The unique feature of all stem cells, including HSCs, is their exceptional ability to divide asymmetrically giving rise to two different kinds of offspring. One daughter cell becomes an LT-HSC itself (self-renews) to maintain the LT-HSC pool, whereas the second daughter cell pursues a differentiation fate to ultimately give rise to terminally differentiated mature blood cells (Orkin and Zon, 2008). Quantification of phenotypic LT-HSCs can be performed by multi-color flow cytometry and the gold standard for assessment of LT-HSC self-renewal and function is competitive bone marrow transplantation (Miller et al. ... [摘要]  造血分化是一种非常复杂的过程,源自称为长期重建造血干细胞(LT-HSCs)的非凡细胞群。所有干细胞(包括HSC)的独特之处在于其不对称的划分能够产生两种不同种类的后代。一个子细胞成为LT-HSC本身(自我更新)以维持LT-HSC库,而第二个子细胞追求分化命运,最终产生终末分化的成熟血细胞(Orkin和Zon,2008)。表型LT-HSC的定量可以通过多色流式细胞术进行,用于评估LT-HSC自我更新和功能的黄金标准是竞争性骨髓移植(Miller等,2008)。尽管这些方法对于确定LT-HSC丰度和功能是不可替代的,但它们具有其缺点和局限性。例如,竞争性骨髓移植通常被监测为12-16周内外周血献血者贡献的函数。虽然减少的外周血供体贡献本身表示干/祖细胞隔室中的损伤,但是它不能明确区分降低的LT-HSC自我更新,受损的LT-HSC分化或受损的祖细胞分化。这里我们描述一种LT-HSCs甲基纤维素集落形成测定法,作为直接评估LT-HSC分化能力的快速互补体外方法。如Kerenyi等人所述(2013),该技术是区分LT-HSC或祖细胞分化缺陷的有力工具。

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