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Precision cover glasses thickness No. 1.5H, 24 x 50 mm

Company: Marienfeld-Superior
Catalog#: 0107222
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Whole-seed Immunolabeling of Arabidopsis Mucilage Polysaccharides
[Abstract]  In addition to synthesizing and secreting copious amounts of pectic polymers (Young et al., 2008), Arabidopsis thaliana seed coat epidermal cells produce small amounts of cellulose and hemicelluloses typical of secondary cell walls (Voiniciuc et al., 2015c). These components are intricately linked and are released as a large mucilage capsule upon hydration of mature seeds. Alterations in the structure of minor mucilage components can have dramatic effects on the architecture of this gelatinous cell wall. The immunolabeling protocol described here makes it possible to visualize the distribution of specific polysaccharides in the seed mucilage capsule. [摘要]  除了合成和分泌大量的果胶聚合物(Young等人,2008)外,拟南芥种皮表皮细胞产生少量的二级纤维素和半纤维素细胞壁(Voiniciuc等,,2015c)。这些组分复杂连接,并在成熟种子水合时作为大胶囊释放。在较小的粘液组分的结构中的改变可以对该凝胶状细胞壁的结构产生显着的影响。这里描述的免疫标记方案使得可以可视化种子胶囊中特定多糖的分布。

背景 自从拟南芥种皮表皮细胞(Young等人,2008)第一次富含果胶的胶质综合免疫荧光分析以来,在这种特殊的细胞壁中已经检测到另外类型的多糖(Voinicucucum,等等。,2015a; 2015b和2015c)。为了平行处理更多的样本,我修改了原始方案(在1.5 ml微量离心管中执行; Young等人,2008; Harpaz-Saad等人,2011年)到24孔板格式。我建议用Pontamine S4B(一种比以前的污渍更具体的纤维素荧光染料)来重新研磨种子(Anderson等人,2010)。通过测试多个荧光团之间的串扰,并为图像采集和处理设定明确的指导,该方案产生可重复的粘液表型,可以可靠地解释。