{{'Search' | translate}}
 

Bovine Serum Albumin Fraction V

Company: Divbio Science
Catalog#: 41-903-100
Bio-protocol()
Company-protocol()
Other protocol()

Protein Localization in the Cyanobacterium Anabaena sp. PCC7120 Using Immunofluorescence Labeling
Author:
Date:
2017-06-05
[Abstract]  Techniques such as immunoflorescence are widely used to determine subcellular distribution of proteins. Here we report on a method to immunolocalize proteins in Anabaena sp. PCC7120 with fluorophore-conjugated antibodies by fluorescence microscopy. This method improves the permeabilization of cyanobacterial cells and minimizes the background fluorescence for non-specific attachments. In this protocol, rabbit antibodies were raised against the synthetic peptide of CyDiv protein (Mandakovic et al., 2016). The secondary antibody conjugated to the fluorophore Alexa488 was used due to its different emission range in comparison to the autofluorescence of the cyanobacterium. [摘要]  诸如免疫荧光的技术被广泛用于确定蛋白质的亚细胞分布。在这里我们报告一种免疫定位蛋白质的方法。 PCC7120通过荧光显微镜检测荧光团结合的抗体。该方法改善了蓝细菌细胞的透化性,并使非特异性附着物的背景荧光最小化。在该方案中,针对CyDiv蛋白质的合成肽(Mandakovic等人,2016)产生兔抗体。使用与荧光团Alexa488缀合的二抗,因为与蓝细菌的自发荧光相比,其发射范围不同。

背景 蓝细菌的免疫荧光已广泛用于细胞鉴定和计数研究(Jin等人,2016)。然而,在蓝细菌中蛋白质的免疫定位尚未有效地实现。定位蛋白质最复发的方法是通过将感兴趣的蛋白质融合到具有不同发射波长的荧光蛋白(绿色荧光蛋白)(与蓝细菌自发荧光相比))以及随后使用落射荧光或共聚焦显微镜(Flores < em,et="" al。,2016;="">
&NBSP;蓝细菌细胞的结构性质是应用免疫荧光技术的主要挑战。它们由内膜(IM),肽聚糖层(PG)和外膜(OM)组成(Rippka,1988; Baulina,2012; Jin等人,2016),附加外多糖层(鞘)。鞘细胞均存在于单细胞和丝状蓝细菌中(Kehr and Dittmann,2015),其厚度,组成和外观取决于生长条件,代谢状态,细胞分化及其他外部和内部参数(Jin et al。 ...

Comments