| Pituitary Isograft Transplantation in Mice
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Author:
Date:
2017-06-05
[Abstract] The mouse pituitary isograft is a technique developed to administer persistent hormone stimulation, thereby increasing cellular proliferation in the mammary tissue (Christov et al., 1993). The pituitary isograft procedure was first described in ‘Induction of Mammary Cancer in Mice without the Mammary Tumor Agent by Isografts of Hypophyses’ by O. Mühlbock and L. M. Boot in 1959 (Muhlbock and Boot, 1959). Since then, the procedure has seen wide use. A pituitary gland is harvested posthumously from a donor mouse and implanted under the renal capsule of the recipient mouse through a small abdominal incision just below the last rib. Once the pituitary gland is implanted, it begins releasing hormones. These secretions increase serum levels of multiple hormones including prolactin, ...
[摘要] 小鼠垂体同种异体移植是开发用于施用持续激素刺激的技术，从而增加乳腺组织中的细胞增殖（Christov等人，1993）。垂体同种异体移植手术首先在O.Mühlbock和L.M. Boot于1959年（Muhlbock和Boot，1959）的“Inction of Mammary Cancer in Mice without the Mammary Tumor Agent by Isoplagen of Hypophyses”中被描述。此后，程序得到广泛的应用。从供体小鼠后期收获垂体腺，并通过位于最后肋骨正下方的小腹切口植入受体小鼠的肾囊下。脑垂体植入后，开始释放激素。这些分泌物增加多种激素的血清水平，包括催乳素，孕酮和17β-雌二醇（Christov等人，1993）。尽管这些激素对癌细胞增殖，生长，分化和寿命的影响没有被很好地表征，并且在一些情况下是有争议的，垂体同种移植物的净效应是根据应变特异性增加鼠乳腺组织的增殖特征（Lydon等人，1999）。
＆NBSP;下面是描述如何进行垂体同种异体移植手术的协议。经过许多步骤，时间参考列在括号中。每个引用对应于该过程的嵌入式视频中的时间点。 （视频1）
背景 ...
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| Whole Mammary Gland Transplantation in Mice Protocol
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Author:
Date:
2017-06-05
[Abstract] Whole Mammary Gland Transplantation involves transplanting an excised mammary gland into another, more suitable host. This method can be used to extend the life of a mammary gland past the mouse’s life span by transplanting the mammary gland of an older mouse into a young healthy mouse. As you can see in the video below (Video 1), by attaching it to the abdomen of the mouse, the gland will receive a steady blood supply and both epithelial and stromal cells will remain viable for up to one year. Although this method is not used often, it has been part of several experiments including determining whether the stroma or epithelium is the primary target in chemically induced mouse mammary tumorigenesis (Medina and Kittrell, 2005). To monitor transplants, palpate every week for tumor formation. ...
[摘要] 全乳腺移植涉及将切除的乳腺移植到另一个更合适的宿主中。该方法可用于通过将老鼠的乳腺移植到年轻的健康小鼠中来延长乳腺的寿命，超过老鼠的寿命。在下面的视频（视频1）中可以看到，通过将其附加到鼠标的腹部，腺体将获得稳定的血液供应，并且上皮细胞和基质细胞将保持活力长达一年。虽然这种方法不经常使用，但它已经是几个实验的一部分，包括确定基质或上皮是化学诱导的小鼠乳腺肿瘤发生的主要靶标（Medina和Kittrell，2005）。为了监测移植物，每周触诊肿瘤形成。移植的乳腺也可以每8-10周连续传播一次。将移植腺保持在同一只老鼠不超过一年。
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