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Author:
Date:
2017-06-05
[Abstract] Whole Mammary Gland Transplantation involves transplanting an excised mammary gland into another, more suitable host. This method can be used to extend the life of a mammary gland past the mouse’s life span by transplanting the mammary gland of an older mouse into a young healthy mouse. As you can see in the video below (Video 1), by attaching it to the abdomen of the mouse, the gland will receive a steady blood supply and both epithelial and stromal cells will remain viable for up to one year. Although this method is not used often, it has been part of several experiments including determining whether the stroma or epithelium is the primary target in chemically induced mouse mammary tumorigenesis (Medina and Kittrell, 2005). To monitor transplants, palpate every week for tumor formation. ...
[摘要] 全乳腺移植涉及将切除的乳腺移植到另一个更合适的宿主中。该方法可用于通过将老鼠的乳腺移植到年轻的健康小鼠中来延长乳腺的寿命，超过老鼠的寿命。在下面的视频（视频1）中可以看到，通过将其附加到鼠标的腹部，腺体将获得稳定的血液供应，并且上皮细胞和基质细胞将保持活力长达一年。虽然这种方法不经常使用，但它已经是几个实验的一部分，包括确定基质或上皮是化学诱导的小鼠乳腺肿瘤发生的主要靶标（Medina和Kittrell，2005）。为了监测移植物，每周触诊肿瘤形成。移植的乳腺也可以每8-10周连续传播一次。将移植腺保持在同一只老鼠不超过一年。
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