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Author:
Date:
2017-05-20
[Abstract] Eukaryotic nuclear DNA wraps around histone proteins to form a nucleosome, a basic unit of chromatin. Posttranslational modification of histones plays an important role in gene regulation and chromosome duplication. Some modifications are quite stable to be an epigenetic memory, and others exhibit rapid turnover or fluctuate during the cell cycle. Histone H4 Lys20 monomethylation (H4K20me1) has been shown to be involved in chromosome condensation, segregation, replication and repair. H4K20 methylation is controlled through a few methyltransferases, PR-Set7/Set8, SUV420H1, and SUV420H2, and a demethylase, PHF8. In cycling cells, the level of H4K20me1 increases during G2 and M phases and decreases during G1 phase. To monitor the local concentration and global fluctuation of histone ...
[摘要] 真核核DNA包裹组蛋白，形成核小体，是染色质的基本单位。组蛋白的翻译后修饰在基因调控和染色体重复中起重要作用。一些修饰是相当稳定的，作为表观遗传记忆，其他修饰在细胞周期中表现出快速更替或波动。组蛋白H4 Lys20单甲基化（H4K20me1）已显示参与染色体凝聚，分离，复制和修复。通过几种甲基转移酶PR-Set7 / Set8，SUV420H1和SUV420H2以及脱甲基酶PHF8控制H4K20甲基化。在循环细胞中，H4K20me1的水平在G2期和M期增加，G1期下降。为了监测活细胞中组蛋白修饰的局部浓度和全局波动，我们开发了一种基因编码的探针，称为薄荷素（修饰特异性细胞内抗体; Sato等人，2013和2016）。通过测量核细胞与细胞质的强度比，可以监测单个细胞中H4K20me1的相对水平。该详细方案允许甲基转移酶对基于HatoK等人的质粒H4K20me1-mintbody活性细胞中H4K20me1水平的影响进行半定量分析（2016）。
背景 ...
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