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Sodium carbonate AnalaR NORMAPUR® ACS, Reag. Ph. Eur. pour analyses

Company: VWR
Catalog#: 27771.29
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Exopolysaccharide Quantification for the Plant Pathogen Ralstonia solanacearum
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2017-05-20
[Abstract]  Soluble exopolysaccharide is a major virulence factor produced by the plant pathogen Ralstonia solanacearum. Its massive production during plant infection is associated with the arrest of water flow in xylem vessels leading eventually to plant death. The composition of this heavy macromolecule includes mainly N-acetylgalactosamine. Here we describe a colorimetric method for quantitative determination of the soluble exopolysaccharide present in culture supernatant of R. solanacearum. [摘要]  可溶性外多糖是由植物病原体Ralstonia solanacearum产生的主要毒力因子。植物感染期间的大量生产与木质部血管中的水流停滞有关,最终导致植物死亡。该重大分子的组成主要包括N-乙酰半乳糖胺。这里我们描述了用于定量测定R中培养上清液中可溶性外多糖的比色法。雷尔氏菌。

背景 植物病原体罗勒氏菌(Ralstonia solanacearum)在群体感应系统的控制下产生胞外多糖,即高细胞密度,高于5×10 7细胞ml -1 (Flavier等人,1997)。外多糖的糖含量包括比例为10:2.5:1的半乳糖胺,葡萄糖和鼠李糖(Drigues等人,1985)。 Brumbley和Denny(1990)最初开发了用于从培养上清液中可靠地提取和定量外多糖的方案,并且最近由Peyraud等人(2016)更新。定量基于使用适应的Elson和Morgan测定法测定大分子的己糖胺含量(Elson和Morgan,1933; Gatt和Berman,1966)。含有N-乙酰基-D-半乳糖胺的外多糖由不同的革兰氏阴性或革兰氏阳性细菌(Vaningelgem等人,2004; Balzaretti等人,2017)产生,以及一些真菌(Lee等人,2015),因此该方案也可适用于这些生物。

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