Author:
Date:
2017-05-20
[Abstract] A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining, Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, enzyme-linked immunosorbent assay (ELISA), luminex assays, etc., to examine the immune cells, pathogens, proteins such as cytokines/chemokines, and the expression levels of inflammation-related and other genes in the cells. This will help to understand the underlying mechanisms of these lung diseases and develop specific and effective drugs.
[摘要] 这里描述了小鼠中基本的支气管肺泡灌洗(BAL)程序。可以通过Hema3染色,免疫染色,荧光激活细胞分选(FACS),PCR,二金鸡宁酸蛋白测定,酶联免疫吸附测定(ELISA),luminex检测等来分析从BAL获得的细胞和液体。 / em>,以检查免疫细胞,病原体,蛋白质如细胞因子/趋化因子,以及细胞中炎症相关基因和其他基因的表达水平。这将有助于了解这些肺部疾病的潜在机制,并开发具体有效的药物。
背景 支气管肺泡灌洗(BAL)是通常用于诊断肺部疾病(包括肺癌)的简单且典型的方法(Daubeuf和Frossard,2012)。它用于采样肺组分,以确定肺中的蛋白质组成,免疫细胞和病原体。肺部慢性炎症在肺癌起始和进展中起关键作用。为了阐明肺肿瘤发生的炎症的潜在机制,我们的实验室使用了一种基本的BAL方案来确定肺部免疫反应(Qu等人,2015; Zhou等)。 ,2015; Sun等人,2016; Zhou等人,2017)。
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