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Author:
Date:
2017-05-05
[Abstract] To advance the understanding of microbial interactions, it is becoming increasingly important to resolve the individual metabolic contributions of microorganisms in complex communities. Organisms from biofilms can be especially difficult to separate, image and analyze, and methods to address these limitations are needed. High resolution imaging secondary ion mass spectrometry (NanoSIMS) generates single cell isotopic composition measurements, and can be used to quantify incorporation and exchange of an isotopically labeled substrate among individual organisms. Here, incorporation of cyanobacterial extracellular organic matter (EOM) by members of a cyanobacterial mixed species biofilm is used as a model to illustrate this method. Incorporation of stable isotope labeled (15N and 13 ...
[摘要] 为了提高对微生物相互作用的理解，解决复杂群落中微生物代谢贡献的重要性越来越重要。生物膜的生物体特别难以分离，形成和分析，需要解决这些局限性的方法。高分辨率成像二次离子质谱（NanoSIMS）产生单细胞同位素组成测量，可用于量化在各生物体内同位素标记的底物的掺入和交换。在这里，使用由蓝藻混合物种生物膜的成员掺入的蓝细菌胞外有机物（EOM）作为模型来说明该方法。通过两组（蓝细菌和相关的异养微生物）掺入稳定同位素（ 15 N和 13 C）EOM的量化。描述了用于定量生物膜中稳定同位素标记的EOM摄取的样品的生成，制备和分析方法。
背景 与NanoSIMS（“NanoSIP”）结合的稳定同位素标记是一种确定的稳定同位素标记底物掺入到单个微生物细胞中的方法，然后可将其推断以估计细胞群体的掺入（例如，Lechene et al。 ，2006和Woebken等人，2012）。可以将多个稳定同位素标记（例如， 13 C和 15 ...
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