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Sodium Phosphate Monobasic Anhydrous (USP), Fisher Chemical
Company: Fisher Scientific
Catalog#: S397-500
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Escherichia coli Infection of Drosophila
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2017-05-05
[Abstract]  Following septic insults, healthy insects, just like vertebrates, mount a complex immune response to contain and destroy pathogens. The failure to efficiently clear bacterial infections in immuno-compromised fly mutants leads to higher mortality rates which provide a powerful indicator for genes with important roles in innate immunity. The following protocol is designed to reproducibly inject a known amount of non-pathogenic E. coli into otherwise sterile flies and to measure the survival of flies after infection. The protocol can be easily adapted to different types of bacteria. [摘要]  在败血症后，健康的昆虫就像脊椎动物一样，会发生复杂的免疫反应，以遏制和破坏病原体。在免疫损害的蝇突变体中未能有效地清除细菌感染导致更高的死亡率，这为在先天免疫中具有重要作用的基因提供了强有力的指标。以下协议被设计为可重复地注射已知量的非致病性E。大肠杆菌进入其他无菌苍蝇，并测量感染后苍蝇的存活率。该方案可以轻松适应不同类型的细菌。

背景 经典的感染实验包括口服感染果蝇（Chakrabarti等人，2016）或用浸在浓缩细菌溶液中的针（Romeo和Lemaitre，2008）。与这些方案不同，我们的实验程序允许我们确定感染部位，并精确控制注射到每只苍蝇中的细菌的剂量。这提供了均匀性和重复性，并且允许我们适应不同实验的细菌负荷（Akbar等人，2011和2016）。

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