| Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation
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Author:
Date:
2021-03-05
[Abstract] Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for performing this procedure in mouse.
[摘要] [摘要]经盐水灌注心内膜灌注4％多聚甲醛（PFA ）被广泛用于清除血液和保存脑部以进行免疫染色或原位杂交。PFA在溶液中分解成甲醛，甲醛使蛋白质和DNA分子交联以保留组织和细胞结构。在这里，我们提供了在鼠标中执行此过程的逐步指南。
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| Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation
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Author:
Date:
2021-01-05
[Abstract] Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for performing this procedure in mouse.
[摘要] [摘要]经盐水灌注心内膜灌注4％多聚甲醛（PFA ）被广泛用于清除血液和保存脑部以进行免疫染色或原位杂交。PFA在溶液中分解成甲醛，甲醛使蛋白质和DNA分子交联以保留组织和细胞结构。在这里，我们提供了在鼠标中执行此过程的逐步指南。
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| Embryonic Intravitreous Injection in Mouse
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Author:
Date:
2018-07-20
[Abstract] Axons of retinal ganglion cells (RGCs) relay visual information from the retina to lateral geniculate nucleus (LGN) and superior colliculus (SC), which are two major image-forming visual nuclei. Wiring of these retinal projections completes before vision begins. However, there are few studies on retinal axons at embryonic stage due to technical difficulty. We developed a method of embryonic intravitreous injection of dyes in mice to visualize retinal projections to LGN and SC. This study opens up the possibility of understanding early visual circuit wiring in mice embryos.
[摘要] 视网膜神经节细胞（RGC）的轴突将视觉信息从视网膜传递到外侧膝状核（LGN）和上丘（SC），这是两个主要的成像视觉核。 在视力开始之前完成这些视网膜投射的布线。 然而，由于技术困难，很少有关于胚胎期视网膜轴突的研究。 我们开发了一种胚胎玻璃体内注射染料的方法，用于观察LGN和SC的视网膜投射。 这项研究开辟了理解小鼠胚胎早期视觉电路布线的可能性。
【背景】早在胚胎第14.5天（E14.5）小鼠中，视网膜轴突开始向LGN和SC投射。 为了研究胚胎中的轴突投射，需要在注射手术前保持胚胎存活至少10小时，玻璃内注射染料。 以前的研究表明，胚胎可以使用含氧小鼠血清进行体外培养。 然而，营养多样性，氧饱和度和温度调节等问题会影响胚胎的生理状况。 在我们的研究中，我们通过胚胎中的子宫壁和眼睑进行玻璃体内注射，并在注射后将它们保存在子宫中。 从E15.5到E18.5很好地标记了对LGN和SC的视网膜轴突投射。
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