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Author:
Date:
2015-06-05
[Abstract] Despite its extensive use as a nitrogen fertilizer, the role of urea as a directly accessible nitrogen source for crop plants is still poorly understood. So far, the physiological and molecular aspects of urea acquisition have been investigated only in a few plant species highlighting the importance of a urea transporter in roots, DUR3 (Kojima et al., 2007; Wang et al., 2012; Zanin et al., 2014a). Regarding maize plants, a crop that needs a large amount of urea fertilizer, the capability to take up urea via an inducible and high-affinity transport system has been recently characterized (Zanin et al., 2014a; Zanin et al., 2014b). Here, we described a small-scale protocol suitable for the measurement of urea net high-affinity uptake in roots of ...
[摘要] 尽管其作为氮肥的广泛使用，但是作为直接可及的作物植物氮源的脲的作用仍然知之甚少。 迄今为止，仅在少数植物物种中研究了尿素获取的生理学和分子学方面，突出了根中的尿素转运蛋白DUR3的重要性（Kojima等人，2007; et al。，2012; Zanin et al。，2014a）。 关于玉米植物，需要大量尿素肥料的作物，通过诱导型和高亲和力转运系统摄取尿素的能力最近已被表征（Zanin等人，2014a; Zanin et al。，2014b）。 在这里，我们描述了一个小规模的协议，适合测量尿素净高亲和力摄取完好的玉米植物的根。
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