{{'Search' | translate}}
 

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade

Company: Abcam
Catalog#: ab5103
Bio-protocol()
Company-protocol()
Other protocol()

In vitro Detection of Neutrophil Traps and Post-attack Cell Wall Changes in Candida Hyphae
Author:
Date:
2017-04-05
[Abstract]  In this protocol we describe how to visualize neutrophil extracellular traps (NETs) and fungal cell wall changes in the context of the coculture of mouse neutrophils with fungal hyphae of Candida albicans. These protocols are easily adjusted to test a wide array of hypotheses related to the impact of immune cells on fungi and the cell wall, making them promising tools for exploring host-pathogen interactions during fungal infection. [摘要]  在本协议中,我们描述了如何在小鼠嗜中性粒细胞与真菌白色念珠菌的真菌菌丝共培养的背景下观察嗜中性粒细胞胞外捕获物(NETs)和真菌细胞壁变化。 这些方案很容易调整,以测试与免疫细胞对真菌和细胞壁的影响有关的各种假设,使其成为探索真菌感染期间宿主病原体相互作用的有前景的工具。

C。白色念珠菌是一种多态性机会性酵母,嗜中性粒细胞是免疫细胞,对于这种和其他真菌病原体的防御至关重要(Brown等,2012; Lionakis和Netea,2013)。 NETs是可以针对病原体部署的潜在防御机制,并且已经表明它们优先针对诸如C的微生物细胞部署。白念珠菌菌丝太大而不能吞噬(Urban et al。,2006; Bruns等人,2010; Branzk等人,,2014; Rohm等人,2014)。 NET已经显示含有许多组分,包括髓过氧化物酶,胞外DNA和瓜氨酸化的组蛋白(Amulic等人,2012; ...

Comments