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LiChrospher 100 RP-8 5 µm endcapped HPLC column

Company: Trentec
Catalog#: 124LP-85ER
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Assays for the Detection of Rubber Oxygenase Activities
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2017-03-20
[Abstract]  Microbial biodegradation of rubber relies on extracellular rubber oxygenases that catalyze the oxidative cleavage of the double bond of the polyisoprene backbone into oligo-isoprenoids. This protocol describes the determination of rubber oxygenase activities by an online measurement of molecular oxygen consumption via a non-invasive fluorescence-based assay. The produced oligo-isoprenoid cleavage products with terminal keto- and aldehyde-groups are identified qualitatively and quantitatively by HPLC. Our method allows for the characterization of homologue rubber oxygenases, and can likely be adapted to assay other oxygenases consuming dioxygen. Here we describe the determination of rubber oxygenase activities at the examples of the so far two known types of rubber oxygenases, ... [摘要]  橡胶的微生物生物降解依赖于细胞外橡胶加氧酶,其催化聚异戊二烯骨架的双键氧化裂解成低聚异戊二烯。该方案描述了通过基于非侵入性荧光的测定在线测量分子氧消耗来测定橡胶加氧酶活性。通过HPLC定性和定量鉴定产生具有末端酮基和醛基的寡聚异戊二烯切割产物。我们的方法允许表征同系物橡胶加氧酶,并且可能适用于测定消耗双氧的其他加氧酶。在这里,我们描述了迄今为止两种已知类型的橡胶加氧酶,即橡胶加氧酶A(RoxA)和胶乳清除蛋白(Lcp)的实例的橡胶加氧酶活性的测定。

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