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Author:
Date:
2017-03-20
[Abstract] Dipeptidylpeptidases (DPPs) are serine proteases, which cleave small proteins and peptides possessing a proline or an alanine in the second position of their N-terminus. Among the members of this family, dipeptidylpeptidase 4 (DPP4) is constitutively expressed in the extracellular space. DPP4 is found at the surface of many hematopoietic and non-hematopoietic cells and is also present in many biological fluids in a bioactive soluble form. DPP4 expression is modulated by inflammation, and measurements of its activity have been used as biomarker for disease. Here, we describe a method to evaluate the enzymatic activity of DPP4 in vitro and in vivo.
[摘要] 二肽基肽酶（DPPs）是丝氨酸蛋白酶，其在其N末端的第二位置切割具有脯氨酸或丙氨酸的小蛋白质和肽。在该家族成员中，二肽基肽酶4（DPP4）在细胞外空间中组成型表达。 DPP4存在于许多造血细胞和非造血细胞的表面，并且也以生物活性可溶形式存在于许多生物流体中。 DPP4表达受炎症调节，其活性的测定已被用作疾病的生物标志物。在这里，我们描述了一种评估体外DPP4和/或体内酶活性的方法。
背景 DPPs的酶活性的大小可以是炎症的指标;以及用于DPP4抑制剂的重要药效学参数。在这里，我们报告了市售试剂盒用于评估体外DPP-酶活性的详细信息;并提供测试生物样品生成的细节。此外，我们描述了该方法如何用于体内DPP活性的评估。
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