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Author:
Date:
2015-06-05
[Abstract] Almost all individuals infected with human immunodeficiency virus (HIV) are also infected with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have included characterizing and measuring the latent HIV reservoir and understanding the association between asymptomatic replication of CMV (and other herpesvirus, including EBV) and this HIV reservoir (Gianella et al., 2014). This protocol was designed to simultaneously co-extract DNA and RNA from the same peripheral blood mononuclear cell (PBMC) aliquot and quantify HIV, CMV and EBV DNA, as well as HIV RNA using droplet digital PCR (ddPCR).
For collection and processing of male genital secretions and quantification of HIV RNA and DNA from seven human herpesviruses from seminal plasma, refer to ...
[摘要] 几乎所有感染人免疫缺陷病毒（HIV）的个体也被巨细胞病毒（CMV）和EB病毒（EBV）感染。 我们的研究的目的包括表征和测量潜伏的HIV库并且理解CMV（和其他疱疹病毒，包括EBV）的无症状复制与该HIV库之间的关联（Gianella等人，2014） 。 该方案设计为从相同的外周血单核细胞（PBMC）等分试样同时共提取DNA和RNA，并使用液滴数字PCR（ddPCR）定量HIV，CMV和EBV DNA以及HIV RNA。
关于男性生殖器分泌物的收集和处理以及来自七种人疱疹病毒的HIV RNA和DNA的定量，参见方案"Vacs RNA和人疱疹病毒DNA在Seminal血浆中的定量（Vargas-Meneses等人，2015）。
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