{{'Search' | translate}}
 

Thomas Wiley® Mini-Mill Cutting Mill, 115V, 60Hz

Company: Thomas Scientific
Catalog#: 3383L10
Bio-protocol()
Company-protocol()
Other protocol()

Acetyl Bromide Soluble Lignin (ABSL) Assay for Total Lignin Quantification from Plant Biomass
Author:
Date:
2017-03-05
[Abstract]  Lignin is the second most abundant biopolymer on Earth, providing plants with mechanical support in secondary cell walls and defense against abiotic and biotic stresses. However, lignin also acts as a barrier to biomass saccharification for biofuel generation (Carroll and Somerville, 2009; Zhao and Dixon, 2011; Wang et al., 2013). For these reasons, studying the properties of lignin is of great interest to researchers in agriculture and bioenergy fields. This protocol describes the acetyl bromide method of total lignin extraction and quantification, which is favored among other methods for its high recovery, consistency, and insensitivity to different tissue types (Johnson et al., 1961; Chang et al., 2008; Moreira-Vilar et al., 2014; Kapp et al., ... [摘要]  木质素是地球上第二大丰富的生物聚合物,为植物在二次细胞壁提供机械支持,防止非生物和生物胁迫。然而,木质素还可以作为生物燃料生成的糖化的障碍(Carroll和Somerville,2009; Zhao和Dixon,2011; Wang等人,2013)。由于这些原因,研究木质素的性质对于农业和生物能源领域的研究人员非常感兴趣。该方案描述了总木素提取和定量的乙酰溴方法,其对于其不同组织类型的高回收率,一致性和不敏感性的其它方法是有利的(Johnson等人,1961; Chang < em等人,2008;="" moreira-vilar等人,2014;="">

背景 用于从植物生物量定量木质素的乙酰溴方法已被用于精确测量几十年的总木质素含量(Johnson等,1961)。最近,与替代的巯基乙酸和Klason木质素方法(Moreira-Vilar等人,2014))相比,该方法获得了木质素定量的最佳方法的支持。这三种方法的比较经验证明,乙酰溴方法一直导致木质素回收率最高,对组织类型,木质化程度和木质素组成不敏感(Moreira-Vilar等人)。 ...

Comments