{{'Search' | translate}}
 

Synergy® Water Purification System

Company: EMD Millipore
Catalog#: SYNS0HFWW
Bio-protocol()
Company-protocol()
Other protocol()

An HPLC-based Method to Quantify Coronatine Production by Bacteria
Author:
Date:
2017-03-05
[Abstract]  Coronatine is a polyketide phytotoxin produced by several pathovars of the plant pathogenic bacterium Pseudomonas syringae. It is one of the most important virulence factors determining the success of bacterial pathogenesis in the plant at both epiphytic and endophytic stages of the disease cycle. This protocol describes an optimized procedure to culture bacterial cells for coronatine production and to quantify the amount of coronatine secreted in the culture medium using an HPLC-based method. [摘要]  冠心病是由植物病原菌的几种病原体产生的聚酮化合物植物毒素。它是在疾病周期的附生和内生期阶段确定植物细菌发病机制成功的最重要的毒力因素之一。该方案描述了用于培养用于冠状病毒产生的细菌细胞的优化方法,并且使用基于HPLC的方法定量培养基中分泌的冠状动脉的量。

背景 Coronatine(COR)是有效的细菌植物毒素,是植物激素茉莉酮酸-L异亮氨酸(JA-Ile)的分子模拟物。因此,COR激活茉莉酸(JA)信号传导,诱导JA响应基因,并拮抗免疫信号水杨酸的作用。 COR由两种成分组成:冠心病(CFA)和冠状氨酸(CMA)。编码CMA和CFA生物合成的基因在细菌中不是组成型表达的。相反,当细菌在诱导培养基中生长时,这些基因在植物叶片表面或植物体内或/或体外诱导(Palmer和Bender,1993; Panchal等等,2016)。本文介绍了从Panchal等人改编的方法。 (2016),以确定细菌产生冠心病的能力,可用作毒性指征。

Comments