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Author:
Date:
2017-02-20
[Abstract] miRNA are short non-coding RNA which inhibit translation of mRNA. miRNA regulate several cellular processes. Certain miRNA are known to induce oncogenesis. miRNA can be measured by real-time PCR and be imaged using a combination of in situ hybridization (ISH) and quantum dots (QD). The advantage of using quantum dots is that several miRNA can be simultaneously measured using multiplexed QD. Additionally, miRNA can be visualized in different regions of the tissue. Since miRNA are biomarkers of various disease states, miRNA can be visualized and quantitated in tissue sections for diagnostic and prognostic purposes. Here we describe ISH-QD analysis of tissue sections. Tissue sections from xenografts or clinical specimens are used. These are deparaffinized, treated with Proteinase K ...
[摘要] miRNA是抑制mRNA翻译的短非编码RNA。 miRNA调节几种细胞过程。已知某些miRNA诱导肿瘤发生。 miRNA可以通过实时PCR测量，并使用原位杂交（ISH）和量子点（QD）的组合进行成像。使用量子点的优点是可以使用多重QD同时测量几种miRNA。此外，miRNA可以在组织的不同区域可视化。由于miRNA是各种疾病状态的生物标志物，miRNA可以在组织切片中进行可视化和定量，以用于诊断和预后。这里我们描述组织切片的ISH-QD分析。使用来自异种移植物或临床标本的组织切片。将其脱石蜡，用蛋白酶K处理并与生物素探针杂交以对miRNA具有特异性。原位杂交通过标记生物素探针进行，然后用链霉亲和素标记的量子点进行标记。进行量子点的图像采集并分析其miRNA表达水平。结合ISH和QD为检测组织不同细胞中的miRNA提供了强大的工具。
背景 ...
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