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Author:
Date:
2017-02-20
[Abstract] Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a quiescent non motile mode. Adhesion interactions between HSCs and their supporting BM microenvironment cells are critical for maintaining stem cell quiescence and protection from DNA damaging agents to prevent hematology failure and death. Multiple signaling proteins play a role in controlling retention and migration of bone marrow HSCs. Adhesion molecules are involved in both processes regulating hematopoiesis and stem- and progenitor-cell BM retention, migration and development. The mechanisms underlying the movement of stem cells from and to the ...
[摘要] 造血干细胞（HSC）由其自我更新的功能能力定义，并分化成所有血细胞谱系。大多数HSC位于骨髓（BM）微环境中的特定解剖位置，处于静止非运动模式。 HSCs与其支持的BM微环境细胞之间的粘附相互作用对于维持干细胞静止和保护免受DNA损伤因子阻止血液学失败和死亡至关重要。多重信号蛋白在控制骨髓HSCs的保留和迁移中起重要作用。粘附分子参与调节造血和干细胞和祖细胞BM保留，迁移和发育的两个过程。干细胞从骨髓移动到骨髓的机制尚未完全阐明，仍然是深入研究的对象。一个重要的方面是修饰干细胞保留，迁移和发育所需的干细胞和祖细胞的粘附分子的表达和亲和力。粘附力通过粘附分子的表达，亲和力和亲合力来调节。亲和力调节与分子结合识别和结合强度有关。在这里，我们描述了在我们的研究中使用的体外 FACS测定来研究整联蛋白α4亚类β1亚单位的表达，亲和力和功能也称为VLA-4）用于小鼠骨髓保留EPCR +长期复制HSC（LT-HSC）（Gur-Cohen等人，2015）。背景整合素是介导细胞和细胞 - ...
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