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TrueLine Cell Culture Dishes

Company: TrueLine
Catalog#: TR4002
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Pseudomonas syringae Flood-inoculation Method in Arabidopsis
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Date:
2017-01-20
[Abstract]  Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we demonstrate a rapid and reliable flood-inoculation method based on young Arabidopsis seedlings grown on one-half strength MS medium. We also describe a method to evaluate the bacterial growth in Arabidopsis. [摘要]  丁香假单胞菌 pv。 由于其在拟南芥中的致病性,已经将用于番茄细菌斑病的番茄株DC3000( DC3000)用作模型病原体 >。 在这里,我们展示了一种基于在一半强度MS培养基上生长的幼苗拟南芥幼苗的快速可靠的接种方法。 我们还描述了一种评估拟南芥细菌生长的方法。

背景 A。 DC3000模型病理学被广泛用于研究微生物发病机制和植物先天免疫的分子机制(Ishiga等,2012和2016; Ishiga和Ichinose,2016)。 虽然已经开发了几种接种方法来研究该模型系统中的相互作用,但迄今报道的方法之一与自然界中发生的方法相似。

Karyotype Analysis
Author:
Date:
2014-05-20
[Abstract]  A chromosome is the structure that organizes DNA and protein in cells. It is a single piece of coiled DNA containing coding and non-coding sequences. Human cells have 23 pairs of chromosomes including 22 pairs of autosomes and one pair of sex chromosome, giving a total of 46 per cell. In tumor cells, chromosomal instability has been considered to be one of the hallmarks of tumor formation. Here we use the karyotype analysis to separate the chromosomes and observe the chromosomes in tumor cells with a microscope.
[摘要]  染色体是组织细胞中的DNA和蛋白质的结构。 它是含有编码和非编码序列的单片卷曲DNA。 人类细胞具有23对染色体,包括22对常染色体和一对性染色体,每个细胞总共46个染色体。 在肿瘤细胞中,染色体不稳定性已被认为是肿瘤形成的标志之一。 这里我们使用核型分析来分离染色体,并用显微镜观察肿瘤细胞中的染色体

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