{{'Search' | translate}}
 

Labo incubator

Company: AS ONE
Catalog#: ICV-300P
Bio-protocol()
Company-protocol()
Other protocol()

Pseudomonas syringae Flood-inoculation Method in Arabidopsis
Author:
Date:
2017-01-20
[Abstract]  Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we demonstrate a rapid and reliable flood-inoculation method based on young Arabidopsis seedlings grown on one-half strength MS medium. We also describe a method to evaluate the bacterial growth in Arabidopsis. [摘要]  丁香假单胞菌 pv。 由于其在拟南芥中的致病性,已经将用于番茄细菌斑病的番茄株DC3000( DC3000)用作模型病原体 >。 在这里,我们展示了一种基于在一半强度MS培养基上生长的幼苗拟南芥幼苗的快速可靠的接种方法。 我们还描述了一种评估拟南芥细菌生长的方法。

背景 A。 DC3000模型病理学被广泛用于研究微生物发病机制和植物先天免疫的分子机制(Ishiga等,2012和2016; Ishiga和Ichinose,2016)。 虽然已经开发了几种接种方法来研究该模型系统中的相互作用,但迄今报道的方法之一与自然界中发生的方法相似。

Comments