{{'Search' | translate}}
 

Acetic Acid

Company: Sangon Biotech
Catalog#: A501931
Bio-protocol()
Company-protocol()
Other protocol()

Chitin Extraction and Content Measurement in Magnaporthe oryzae
Author:
Date:
2017-03-05
[Abstract]  Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as an example. [摘要]  壳多糖是由β(1→4)连接的N-乙酰葡糖胺(GlcNAc)残基组成的线性多糖。在真菌中,几丁质是细胞壁的重要成分。在这里,我们提供了使用稻瘟病菌(Metaporthe oryzae)来测量真菌细胞的壳多糖含量的方案。

背景 壳多糖是真菌病原体细胞壁的重要成分,是众所周知的病原体相关分子模式(PAMP)。确定真菌物种的几丁质含量对于研究真菌生物学和宿主 - 病原体相互作用是重要的。基于比色法的Morgan-Elson方法已经在酵母中调节以测量细胞几丁质水平(Leloir和Cardini,1953; Bulik等人,2003; Baker等人, ,,2007)。然而,没有针对稻米稻瘟病菌确定的具体方案,这是世界上最重要的真菌病的稻瘟病的致病因子。在这里,我们描述了一种可靠和简单的方案,该协议由Morgan-Elson方法修改,以测试M的甲壳质含量。 oryzae (Song 等人,2010)。

Expression and Purification of the GRAS Domain of Os-SCL7 from Rice for Structural Studies
Author:
Date:
2017-02-05
[Abstract]  GRAS proteins, named after the first three members GAI, RGA and SRC, has been found in 294 embryophyta species and is represented by 1,035 sequences. They belong to a plant-specific protein family and play essential roles in plant growth and development. Proteins in this family are defined as minimally containing a conserved GRAS domain, which is about 350-450 resides and can be subdivided into five distinct motifs with their name derived from the most prominent amino acids: LRI (leucine-rich region I), VHIID, LRII (leucine-rich region II), PFYRE and SAW and mainly function in the interaction between GRAS proteins and their partners (Sun et al., 2012).By phylogenetic analysis, the GRAS family can be divided into more than ten subfamilies, of which SCL4/7 is one important subgroup ... [摘要]  以前3个成员GAI,RGA和SRC命名的GRAS蛋白质已被发现在294个胚胎种,并由1,035个序列表示。它们属于植物特异性蛋白质家族,在植物生长和发育中起重要作用。该家族中的蛋白质被定义为最低限度地含有保守的GRAS结构域,其约为350-450个,并且可以细分为五个不同的基序,其名称源自最突出的氨基酸:LRI(富含亮氨酸的区域I),VHIID ,LRII(富含亮氨酸的区域II),PFYRE和SAW,并且主要在GRAS蛋白质与其配偶体之间的相互作用中起作用(Sun等人,2012)。通过系统发育分析,GRAS家族可以分为十多个亚科,其中SCL4 / 7是一个重要的亚组,对应对环境压力有作用。这里我们描述了Os-SCL7(水稻SCL4 / 7成员)的GRAS结构域的表达和纯化的详细方案,使我们能够使其结晶并确定其结构。

背景 GRAS蛋白是一个大家族,在植物发育和信号转导中起重要作用。结果表明,一些家庭成员如DELLAs起到GA响应植物生长的抑制作用,是GA信号通路(Murase等人,2008)中的关键调控目标,NSP1和NSP2起重要作用在调节结瘤发育和信号传导(Kaló等人,2005)中,蛋白质SCR和SHR一起在控制根和芽的径向图案中起重要作用(Helariutta et ...

Comments