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Tris (hydroxymethyl) aminomethane

Company: Sangon Biotech
Catalog#: A100826
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Embryonic Intravitreous Injection in Mouse
Author:
Date:
2018-07-20
[Abstract]  Axons of retinal ganglion cells (RGCs) relay visual information from the retina to lateral geniculate nucleus (LGN) and superior colliculus (SC), which are two major image-forming visual nuclei. Wiring of these retinal projections completes before vision begins. However, there are few studies on retinal axons at embryonic stage due to technical difficulty. We developed a method of embryonic intravitreous injection of dyes in mice to visualize retinal projections to LGN and SC. This study opens up the possibility of understanding early visual circuit wiring in mice embryos. [摘要]  视网膜神经节细胞(RGC)的轴突将视觉信息从视网膜传递到外侧膝状核(LGN)和上丘(SC),这是两个主要的成像视觉核。 在视力开始之前完成这些视网膜投射的布线。 然而,由于技术困难,很少有关于胚胎期视网膜轴突的研究。 我们开发了一种胚胎玻璃体内注射染料的方法,用于观察LGN和SC的视网膜投射。 这项研究开辟了理解小鼠胚胎早期视觉电路布线的可能性。

【背景】早在胚胎第14.5天(E14.5)小鼠中,视网膜轴突开始向LGN和SC投射。 为了研究胚胎中的轴突投射,需要在注射手术前保持胚胎存活至少10小时,玻璃内注射染料。 以前的研究表明,胚胎可以使用含氧小鼠血清进行体外培养。 然而,营养多样性,氧饱和度和温度调节等问题会影响胚胎的生理状况。 在我们的研究中,我们通过胚胎中的子宫壁和眼睑进行玻璃体内注射,并在注射后将它们保存在子宫中。 从E15.5到E18.5很好地标记了对LGN和SC的视网膜轴突投射。

Expression and Purification of the GRAS Domain of Os-SCL7 from Rice for Structural Studies
Author:
Date:
2017-02-05
[Abstract]  GRAS proteins, named after the first three members GAI, RGA and SRC, has been found in 294 embryophyta species and is represented by 1,035 sequences. They belong to a plant-specific protein family and play essential roles in plant growth and development. Proteins in this family are defined as minimally containing a conserved GRAS domain, which is about 350-450 resides and can be subdivided into five distinct motifs with their name derived from the most prominent amino acids: LRI (leucine-rich region I), VHIID, LRII (leucine-rich region II), PFYRE and SAW and mainly function in the interaction between GRAS proteins and their partners (Sun et al., 2012).By phylogenetic analysis, the GRAS family can be divided into more than ten subfamilies, of which SCL4/7 is one important subgroup ... [摘要]  以前3个成员GAI,RGA和SRC命名的GRAS蛋白质已被发现在294个胚胎种,并由1,035个序列表示。它们属于植物特异性蛋白质家族,在植物生长和发育中起重要作用。该家族中的蛋白质被定义为最低限度地含有保守的GRAS结构域,其约为350-450个,并且可以细分为五个不同的基序,其名称源自最突出的氨基酸:LRI(富含亮氨酸的区域I),VHIID ,LRII(富含亮氨酸的区域II),PFYRE和SAW,并且主要在GRAS蛋白质与其配偶体之间的相互作用中起作用(Sun等人,2012)。通过系统发育分析,GRAS家族可以分为十多个亚科,其中SCL4 / 7是一个重要的亚组,对应对环境压力有作用。这里我们描述了Os-SCL7(水稻SCL4 / 7成员)的GRAS结构域的表达和纯化的详细方案,使我们能够使其结晶并确定其结构。

背景 GRAS蛋白是一个大家族,在植物发育和信号转导中起重要作用。结果表明,一些家庭成员如DELLAs起到GA响应植物生长的抑制作用,是GA信号通路(Murase等人,2008)中的关键调控目标,NSP1和NSP2起重要作用在调节结瘤发育和信号传导(Kaló等人,2005)中,蛋白质SCR和SHR一起在控制根和芽的径向图案中起重要作用(Helariutta et ...

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