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Transferpette® S, adjustable volume Single-channel microliter pipette, 100-1,000 μl

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Catalog#: 704780
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In vitro Floral Induction of Cuscuta reflexa
Author:
Date:
2017-01-20
[Abstract]  Floral initiation and development in the angiosperms is the essential step on which the yield of the plant depends. Sometimes external climate or any abiotic stress hinders the floral initiation and ultimately affect the plant yield. Hence, in vitro floral induction and development can help to overcome the external climatic factor. Furthermore, the protocol for in vitro floral induction in the parasitic angiosperm like Cuscuta reflexa has not been reported yet. We have standardized the protocol for floral induction in the parasitic plant Cuscuta reflexa. In this study it is established that MMS (modified Murashige Skoog) media supplemented with 2 mg L-1 NAA (naphthalene acetic acid, plant growth regulator) and 2 mg L-1 2,4-D ... [摘要]  被子植物的花卉引发和发育是植物产量依赖的关键步骤。有时,外部气候或任何非生物胁迫阻碍花卉开始,最终影响植物产量。因此,体外花卉诱导和发育可以帮助克服外部气候因素。此外,尚未报道如在睾丸反射中的寄生被子植物体外花诱导的方案。我们已经规定了寄生植物茧丝反射中花诱导的方案。在本研究中,确定补充有2mg L NAA(萘乙酸,植物生长调节剂)和2mg L -1的MMS(改良的Murashige Skoog)培养基> 2,4-D(2,4-二氯苯氧基丙酸,植物生长调节剂)在体外拍摄后支持花诱导。此外,我们发现补充有2mg L-sup-2,4-D的MMS培养基从淋巴结外植体直接快速诱导花蕾,而没有任何芽伸长。该协议将有助于研究人员在其他被子植物植物中诱导花along与镰刀菌反射。背景 睾丸反射是寄生在大量被子植物上寄生的被子植物(Kuijt,1969)。在宿主植物中造成严重损害和产量损失的能力使得该物种对科学研究非常重要(Nun and Mayer,1999)。除了少数具有功能性叶绿体(Hibberd等人,1998)之外,大多数Cuscuta 物种是非叶绿体。从较大的胚胎开发幼苗。已经报道了白色介质中的反射(Maheshweri和Baldev,1961)。花卉诱导。反射炎在短时间内以及黑暗条件已经在体外中看到(Baldev,1962)。 ...

Pot Level Drought Stress Tolerance Assay in Tobacco through Plant Phenotyping and Antioxidant Assay
Author:
Date:
2015-10-05
[Abstract]  Drought is an important abiotic factor which has a huge detrimental impact on crop productivity. Study of plant responses towards drought stress and investigating the mechanism of drought tolerance is crucial for achieving the target of developing drought-tolerant plants. Phenotyping is a cost effective approach which can be adopted to evaluate the severity of drought stress in a plant. Next to phenotyping parameters, biochemical parameters such as the study of antioxidant enzyme activity play significant roles in assessing the extent of drought stress caused injury in a plant. Among the antioxidant enzymes, ascorbate peroxidase is an enzyme which plays a crucial role in drought tolerance in plants. It has been well established that the activity of this enzyme increases under drought ... [摘要]  干旱是一个重要的非生物因素,对作物生产力有巨大的不利影响。研究植物对干旱胁迫的反应和研究干旱耐受机制对于实现发展耐旱植物的目标是至关重要的。表型是一种成本有效的方法,可用于评价植物中干旱胁迫的严重性。除了表型参数之外,生物化学参数例如抗氧化酶活性的研究在评估植物中干旱胁迫引起的损伤的程度中起重要作用。在抗氧化酶中,抗坏血酸过氧化物酶是在植物的耐旱性中起关键作用的酶。已经确定该酶的活性在干旱胁迫下增加。在这里,我们提出了一个简单和可重复的协议,以调查烟草植物对干旱胁迫通过测量表型参数和抗氧化酶活性的反应。虽然,这些实验已经进行了烟草植物,这个协议可以采用其他作物品种。

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