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Sodium hypochlorite solution

Sodium hypochlorite solution

Company: White King
Catalog#: 10006062A
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Visualising Differential Growth of Arabidopsis Epidermal Pavement Cells Using Thin Plate Spline Analysis
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2016-11-20
[Abstract]  Epidermal pavement cells in Arabidopsis leaves and cotyledons develop from relatively simple shapes to form complex cells that have multiple undulations of varying sizes. Analyzing the growth of individual parts of the cell wall boundaries over time is essential to understanding how pavement cells develop their complex shapes. Thin plate spline analysis is a method for visualizing the change of size and shape of objects through warping or deformation of a regular mesh and can be applied to understand cell wall growth. This protocol describes the application of thin plate spline analysis to visualize the development of individual pavement cells over time. [摘要]  拟南芥叶和子叶中的表皮铺路细胞从相对简单的形状发育而形成具有不同大小的多个起伏的复合细胞。分析细胞壁边界的各个部分随时间的生长对理解铺路细胞如何发展其复杂形状是至关重要的。薄板样条分析是通过规则网格的翘曲或变形来可视化物体的尺寸和形状的变化的方法,并且可以用于理解细胞壁生长。该协议描述了薄板样条分析的应用,以便随时间可视化单个路面细胞的发育。

[背景] 了解细胞生长的空间模式提供了洞察植物细胞如何形成不同的形状。拟南芥子叶和叶的表皮铺路细胞是用于研究复杂细胞如何生长的良好模型系统,因为它们的细胞壁边界从最初为简单弧的边界开始形成不同大小的多个起伏(Armor et al。,2015; Fu et al。,2005)。通过将外部施加的标记物固定到细胞例如藻类氮细胞节间(Green等人,1970),根细胞(Shaw等人, ,2000)和毛状体(Schwab等人,2003)。然而,从外部施加的界标测量细胞生长有时是不可行的,例如当外部施加的荧光标记物的强荧光会遮蔽细胞内荧光标记的细胞骨架元件时(Armor等人,2015)。显示定义数量的同源界标随时间或在不同物体之间的变化位置的薄板样条分析先前已用于分析诸如人类头骨的物体的三维大小和形状的变化(Rosas和Bastir,2002; Gunz ,2014)和叶子(Polder et al ...

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