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Author:
Date:
2015-05-05
[Abstract] Manganese (Mn) is an essential nutrient required for the catalytic or regulatory function of several cellular enzymes. However, excessive Mn concentrations in plant tissues are toxic to plant cells as they negatively affect enzymatic activities, lead to oxidative stress and disturb the uptake and distribution of other essential mineral elements (Ca, P, Mg or Fe). Plants have developed multiple mechanisms to avoid heavy metals (including Mn) toxicity, including transport across the plasma membrane or tonoplast. The genes encoding transporters involved in Mn detoxification are now being identified in different plant species, and functional characterization of genes isolated from species can be easily carried out in Arabidopsis.
Here we provide a method to evaluate the ...
[摘要] 锰（Mn）是几种细胞酶的催化或调节功能所需的必需营养素。然而，植物组织中过量的Mn浓度对植物细胞是有毒的，因为它们对酶活性有负面影响，导致氧化应激并扰乱其他必需矿物元素（Ca，P，Mg或Fe）的摄取和分布。植物已经开发了多种机制以避免重金属（包括Mn）毒性，包括跨质膜或质膜的转运。编码参与Mn解毒的转运蛋白的基因现在在不同的植物物种中被鉴定，并且从物种中分离的基因的功能表征可以在拟南芥中容易地进行。
在这里，我们提供了一种方法来评估用空载体pMDC43或携带黄瓜基因的相同载体 CsMTP8 编码推定的锰转运蛋白的拟南芥线的过量Mn的耐受性定位于液泡膜中。我们分析了在16天期间在含有不同浓度的MnSO 4的无菌平板上在受控条件下生长的植物（植物软体）的生长和发育表型。在相同的植物中测量Mn积累，所述植物在补充有或没有（对照）有毒的Mn浓度的液体培养基中生长
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