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Company: Sigma-Aldrich

Catalog#: E2758

Isolation and Culture of Human Adipose-derived Stem Cells from Subcutaneous and Visceral White Adipose Tissue Compartments

Xiaojia Ge, Shi Chi Leow, Durgalakshmi Sathiakumar, Walter Stünkel, Asim Shabbir, Jimmy Bok Yan So, Davide Lomanto and Craig McFarlane

Published online: 2016-11-20

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Isolation and Primary Culture of Various Cell Types from Whole Human Endometrial Biopsies

Author:

Flavio Santos Vasconcelos Barros, Jan Joris Brosens and Paul John Brighton,

Date:

2016-11-20

[Abstract] The isolation and primary culture of cells from human endometrial biopsies provides valuable experimental material for reproductive and gynaecological research. Whole endometrial biopsies are collected from consenting women and digested with collagenase and DNase I to dissociate cells from the extracellular matrix. Cell populations are then isolated through culturing, filtering and magnetic separation using cell-surface antigen markers. Here we provide a comprehensive protocol on how to isolate and culture individual cell types from whole endometrial tissues for use in in vitro experiments. [摘要] 从人子宫内膜活组织检查中分离和原代培养细胞为生殖和妇科研究提供了有价值的实验材料。从同意的妇女收集完整的子宫内膜活检组织并用胶原酶和DNA酶I消化以从细胞外基质中分离细胞。然后通过使用细胞表面抗原标记物的培养，过滤和磁性分离来分离细胞群。在这里，我们提供了如何从整个子宫内膜组织分离和培养个体细胞类型以用于体外实验的全面方案。

[背景] 人子宫内膜是子宫内层最粘的层。其由柱状上皮和基础基质层组成，其经历循环再生，生长和响应于循环激素的转化。子宫内膜衬里分化为腺分泌表型为囊胚植入和成功怀孕提供了一个好客的环境。在没有怀孕这个层脱落，导致月经。从人子宫内膜活组织检查中分离和培养细胞允许在体外功能评估和与患者结果相关的细胞特征的研究。子宫内膜细胞的分离和培养是研究妇科和产科医学的许多方面的非常宝贵的研究模型，包括不孕，植入失败，复发性流产和月经紊乱。全人子宫内膜活检包含人子宫内膜基质细胞（HESCs），腔和子宫内膜上皮细胞（HEEC），红细胞和免疫细胞的混合群体。 ...

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