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3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4',4''-disulfonic acid sodium salt

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4',4''-disulfonic acid sodium salt

Company: Sigma-Aldrich
Catalog#: 82950
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Hydrogen Peroxide Measurement in Arabidopsis Root Tissue Using Amplex Red
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2016-11-05
[Abstract]  This protocol describes the measurement of hydrogen peroxide (H2O2) content in Arabidopsis root tissue by using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. When root tissue is disrupted and resuspended in phosphate buffer, H2O2 is released from the cells. The obtained root extracts containing H2O2 can be mixed with a solution containing Amplex® Red reagent (10-acetyl-3,7-dihydrophenoxazine). In the presence of horseradish peroxidase, the Amplex® Red reagent reacts with H2O2 in a 1:1 stoichiometry. The resulting product is the red-fluorescent compound resorufin which can be detected fluorometrically or spectrophotometrically. Our protocol is based on ... [摘要]  该方案描述了在拟南芥根组织中通过使用Amplex来测量过氧化氢(H 2 O 2 O 2)含量。 sup>红色过氧化氢/过氧化物酶测定试剂盒。当根组织被破坏并重新悬浮在磷酸盐缓冲液中时,H 2 O 2 O 2从细胞中释放。可以将获得的含有H 2 O 2 O 2的根提取物与含有Amplex Red试剂(10-乙酰基-3,7-二 - 。在辣根过氧化物酶的存在下,Amplex Red试剂与H 2 O 2以1:1的化学计量反应。所得产物是红色荧光化合物试卤灵,其可以通过荧光测定或分光光度法检测。我们的方案是基于Amplex ®红色过氧化氢/过氧化物酶测定试剂盒的手册,并描述了一步一步的程序,详细描述必要的控制和数据分析。我们还包括旨在帮助用户容易地用其自己的样品再现测定的方案,说明和实施例的修改。

[背景] 活性氧(ROS),例如H 2 O 2 O 2可以在该细胞是发育提示或应激条件的结果。在大量,ROS积累可能是有害的,通过造成细胞损伤。然而,增加的ROS产生也可以具有信号传导作用,并且作为控制下游细胞反应的第二信使。在植物中,对于许多非生物胁迫,例如干旱,盐度,温度胁迫和营养缺乏,已经显示ROS的信号传导作用(Mittler,2002; Mittler和Blumwald,2015; Xia等人)。 ,2015)。在我们最近的出版物Le ...

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