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Author:
Date:
2016-11-05
[Abstract] The SRB assay has been used since its development in 1990 (Skehan et al., 1990) to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies (Vichai and Kirtikara, 2006). This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation.
The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient ...
[摘要] Limonium 已知有性和无融合生殖（通过种子的无性繁殖）繁殖模式。在这里，我们提出解剖协议为胚珠的发芽。使用微分干涉对比（DIC）显微镜。该协议允许更好地处理胚珠，并提供优于早期技术，特别是在较大胚珠的某些优势。这种方法还能够观察到完整胚珠中的减数分裂和胚囊发育，以及容易检测到区别性和无序性发育的事件。
[背景] 发生在胚珠发育期间，有必要细胞学检查胚珠。这项研究可以涉及显微镜观察石蜡或树脂包埋，切片材料或清除的器官。在D'Amato（1940; 1949）的先驱作品中公开了对性和无融合生殖物种中胚珠和胚囊发育的第一次细胞学研究。在这些作品中，花使用Karpechenko的方法固定，包埋在石蜡中，切片并用Heidenhain的铁苏木精染色，其染色质和染色体在细胞核中染色。使用这些方法的花芽切片可导致由于单个细胞的部分破坏结构完整性而具有差质量的制备物。更容易的选择是清除福尔马林：乙酸：乙醇固定的器官并用纯的Mayer's hemalum染色（Wallis，1957; Stelly等人，1984）。这种技术需要少得多的时间和劳动，特别是对于通常在卵巢中形成小胚珠的物种，这是 Limonium ...
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