{{'Search' | translate}}
 

Plant container pots, 3 L

Plant container pots, 3 L

Company: National Agrochemical Distributors
Catalog#: POTS34
Bio-protocol()
Company-protocol()
Other protocol()

Assessment of Wheat Resistance to Fusarium graminearum by Automated Image Analysis of Detached Leaves Assay
Author:
Date:
2016-12-20
[Abstract]  Fusarium head blight (FHB) caused by Fusarium pathogens is a globally important cereal disease. To study Fusarium pathogenicity and host disease resistance, robust methods for disease assessment and quantification are needed. Here we describe the procedure of a detached leaves assay emphasizing the image analysis. The protocol provides the different steps of a rapid, automatic and quantitative image analysis to evaluate leaf area infected by Fusarium graminearum. [摘要]  镰孢霉病引起的枯萎病(FHB)病原体是全球重要的谷物疾病。为了研究镰刀菌致病性和宿主抗病性,需要用于疾病评估和定量的强大方法。这里我们描述强调图像分析的分离叶分析的过程。该方案提供了快速,自动和定量的图像分析的不同步骤,以评估禾谷镰孢感染的叶面积。

背景 通过在开花期的视觉评分估计整个植物水平的小麦FHB抗性,这是费力,耗时且需要空间的。因此,已经开发了加速早期植物阶段对FHB抗性的疾病评估的方法,例如种子发芽测定(Browne,2009),胚芽鞘试验(Shin et al。 ,2014),脱叶测定(Browne和Cooke,2004)和幼苗测定(Li等人,2010)。分离叶分析通常用于评估宿主对镰刀菌的反应,并且成功鉴定了FHB抗性的组分(Browne和Cooke,2004)。在这样的测定中,目视评估病原体的建立是耗时且限制精确测量的。最近由Perochon等人描述的方法。 (2015),并在此详细解析了这两个限制,通过使用自动方法,量化基于粒径的图像分析感染的叶面积。

Comments