| Exit from Pluripotency Assay of Mouse Embryonic Stem Cells
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Author:
Date:
2017-08-20
[Abstract] A novel method to assess the dissolution of the core pluripotency transcription-factor circuit of mouse Embryonic Stem Cells (mESCs) has been developed (Ying et al., 2003; Betschinger et al., 2013). In order to efficiently identify genes essential for the break-down of the pluripotency network in mutant mESCs with proliferation defects, we adapted this ‘exit from pluripotency assay’ (Bodak et al., 2017; Cirera-Salinas et al., 2017). The protocol described here has been successfully applied to several mESC lines and is easily transposable from one laboratory to another.
[摘要] 已经开发了评估小鼠胚胎干细胞（mESCs）的核心多能转录因子电路溶解的新方法（Ying等，2003; Betschinger等，2013）。 为了有效识别具有增殖缺陷的突变体mESCs中多能网络分解所必需的基因，我们调整了这种“多能性测定法”（Bodak等，2017; Cirera-Salinas等，2017）。 这里描述的方案已经成功应用于几个mESC系列，并且可以容易地从一个实验室转座到另一个实验室。
【背景】几十年来，科学家已经尝试确定基因与一般（例如胚胎体）或定向（例如，神经元前体细胞）分化方案的mESCs的分化潜能的机制。最近，发现2i培养基允许在体外俘获天真的干细胞（Ying et al。，2008）。 ...
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| Quantitative Determination of Ascorbate from the Green Alga Chlamydomonas reinhardtii by HPLC
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Author:
Date:
2016-12-20
[Abstract] Ascorbate (Asc, also called vitamin C) is of vital importance to the cellular functions of both animals and plants. During evolution, Asc has become one of the most abundant metabolites in seed plants; however, Asc contents in cyanobacteria, green algae and bryophytes are very low. Here we describe a sensitive and reliable HPLC method for the quantitative determination of cellular Asc content in the green alga Chlamydomonas reinhardtii.
[摘要] 抗坏血酸（Asc，也称为维生素C）对动物和植物的细胞功能至关重要。 在进化过程中，Asc已经成为种子植物中最丰富的代谢物之一; 然而，蓝藻，绿藻和苔藓植物中的Asc含量非常低。 在这里，我们描述了一种灵敏可靠的HPLC方法，用于定量测定绿藻衣藻中细胞Asc含量。
背景 针对高等植物开发了用于确定细胞Asc含量的先前方案，其中细胞Asc的量足够高以用于分析。 这些方案不适用于绿色藻类，因为植物材料的量和细胞Asc的水平通常都受到限制。 因此，需要开发一种灵敏度提高到μM范围来测量绿藻中细胞Asc的新方法。
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