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Falcon® 5 mL Round Bottom Polystyrene Test Tube, with Cell Strainer Snap Cap, 25/Pack, 500/Case

5mL Round Bottom Polystyrene Test Tube, with Cell Strainer Snap Cap

Company: Corning
Catalog#: 352235
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In vitro Co-culture of Mesenchymal Stem Cells and Endothelial Colony Forming Cells
Author:
Date:
2017-10-20
[Abstract]  The discovery of endothelial colony forming cells (ECFCs) with robust self-renewal and de novo vessel formation potentials suggests that ECFCs can be an excellent cell source for cardiovascular diseases treatment through improving neovascularization in the ischemic tissues. However, their engraftment after transplantation resulted to be low. Previous studies showed mesenchymal stem/stromal cells (MSCs) could improve the survival and capillary formation capacity of ECFCs in co-culture systems. In this article, we describe a protocol for in vitro co-culture of MSCs and ECFCs to prime ECFCs for better engraftment. [摘要]  发现具有强大自我更新和从头血管形成潜力的内皮细胞集落形成细胞(ECFCs)表明,ECFC可以通过改善缺血组织的新生血管形成,成为心血管疾病治疗的优良细胞来源。 然而,移植后的移植导致了低位移植。 以前的研究显示间充质干/基质细胞(MSC)可以改善共培养系统中ECFCs的存活和毛细管形成能力。 在这篇文章中,我们描述了体外协调MSCs和ECFCs共同培养ECFC以实现更好的移植。
【背景】内皮祖细胞(EPC)被定义为能够通过血管发生过程形成新血管的细胞群。 2004年,Ingram等人鉴定了来自人脐带血的称为“内皮细胞集落形成细胞(ECFC)”的离体培养物中的特异性高度增殖的EPC群体Ingram等人,2004),并且这些细胞最近被宣布代表EPCs(Medina等人,2017)。类似的群体也可以从具有等效血管化潜力和临床相关数量的人类胎盘组织中分离(Patel等人,2013; Shafiee等人,2015) )。因此,ECFC移植已被提出作为缺血性疾病如心肌梗塞或关键性腿部缺血的治疗方法。然而,移植后的ECFCs植入物和血管生成潜力被证明是低的(Shafiee等人,2017; ...

Lipidomic Analysis of Caenorhabditis elegans Embryos
Author:
Date:
2017-09-20
[Abstract]  Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is simple and homogeneous. Here, we demonstrate detailed procedures of C. elegans culture, embryo isolation, lipid extraction and metabolomic data analysis. [摘要]  在心肌梗死(MI)中,许多心肌细胞凋亡。 这些凋亡心肌细胞被吞噬细胞迅速吞噬。 如果死细胞没有被吞没,其有害物质被释放到外面,导致炎症的诱导。 因此,需要去除这些死细胞。 然而,每个吞噬细胞类型对梗塞心脏中凋亡细胞的去除的贡献仍未解决。 在这里,我们描述了吞噬作用测定的体外方案来比较心脏巨噬细胞和心脏肌成纤维细胞的吞噬能力。
【背景】长期以来一直认为,心脏巨噬细胞消除了在失败的心脏中产生的凋亡细胞。 然而,我们发现负责组织纤维化的心脏肌成纤维细胞也具有在MI后吞噬凋亡细胞的能力(Nakaya等,2017)。 这一发现促使我们比较心脏巨噬细胞和心脏肌成纤维细胞的吞噬能力。 在这里,我们提供了一个详细的体外吞噬试验方案来评估吞噬吞噬的程度。

Isolation and Separation of Epithelial CD34+ Cancer Stem Cells from Tgfbr2-deficient Squamous Cell Carcinoma
Author:
Date:
2017-09-05
[Abstract]  Most epithelial tumors have been shown to contain cancer stem cells that are potentially the driving force in tumor progression and metastasis (Kreso and Dick, 2014; Nassar and Blanpain, 2016). To study these cells in depth, cell isolation strategies relying on cell surface markers or fluorescent reporters are essential, and the isolation strategies must preserve their viability. The ability to isolate different populations of cells from the bulk of the tumor will continue to deepen our understanding of the biology of cancer stem cells. Here, we report the strategy combining mechanical tumor dissociation, enzymatic treatment and flow cytometry to isolate a pure population of epithelial cancer stem cells from their native microenvironment. This technique can be useful to further ... [摘要]  大多数上皮肿瘤已经显示含有可能是肿瘤进展和转移的驱动力的癌症干细胞(Kreso和Dick,2014; Nassar和Blanpain,2016)。 为了深入研究这些细胞,依赖于细胞表面标志物或荧光报告基因的细胞分离策略是必不可少的,分离策略必须保持其活力。 从大部分肿瘤中分离不同细胞群的能力将继续加深我们对癌症干细胞生物学的认识。 在这里,我们报告了结合机械肿瘤解离,酶处理和流式细胞术的策略,从其天然微环境中分离出纯种群的上皮癌干细胞。 该技术可用于进一步功能性地分析癌症干细胞(RNA测序和表观遗传学分析),在培养物中培养它们或在移植测定中直接使用它们。
【背景】肿瘤复发和转移是大多数与癌症有关的死亡的主要原因。恶性肿瘤可能由干细胞群体启动和维持(Nassar和Blanpain,2016; Bonnet和Dick,1997),这些细胞是预防复发的重要治疗靶点(Baumann et al。,2008)。研究表明,鳞状细胞癌由肿瘤干细胞亚群维持,其抗药性,并通过进行自我更新和分化(如正常干细胞)引发肿瘤复发,产生增殖祖细胞,其分化形成肿瘤大部分(Locke et al。,2005; Prince et al。,2007; Malanchi et al。,2008; de Sousa e Melo et ...

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