Isolation and Primary Culture of Adult Human Adipose-derived Stromal/Stem Cells
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Author:
Date:
2017-03-05
[Abstract] Adipose-derived stromal/stem cells (ASCs) are multipotent cells that can be isolated from adipose tissue. Studies have shown that cells have the capacity to self-renew and differentiate into adipocyte, chondrocyte, myocyte, and osteoblast lineages. Thus, significant interest regarding their use for regenerative purposes to restore aging or damaged tissue has grown in recent decades. These cells have also been shown to immunomodulate the microenvironment and secrete abundant growth factors, which minimize inflammation and aid repair and regeneration. ASCs can be readily isolated from the stromal vascular fraction (SVF) of lipoaspirates. Given their ease of accessibility, bountiful source, and potential application in regenerative medicine and tissue engineering, there is growing interest ...
[摘要] 脂肪来源的基质/干细胞(ASCs)是可以从脂肪组织分离的多能细胞。研究表明,细胞具有自我更新和分化成脂肪细胞,软骨细胞,肌细胞和成骨细胞谱系的能力。因此,近几十年来,对再生用途恢复老化或损伤组织的兴趣越来越大。这些细胞也被证明可以免疫微环境并分泌丰富的生长因子,从而使炎症最小化并辅助修复和再生。 ASCs可以容易地从脂质体的基质血管分数(SVF)中分离出来。鉴于其易于获取,丰富的来源和在再生医学和组织工程中的潜在应用,对于ASC的表征和利用越来越感兴趣。该方案描述了从成人人类脂肪组织中分离的ASC以及用于培养维持的方法,包括扩增和低温保存。
背景 脂肪来源的基质/干细胞(ASCs)表现出干细胞领域的巨大潜力。根据造血干细胞移植的治疗奇迹,ASCs代表干细胞的未来,因为它们更容易获得源 - 脂肪组织。 ASCs自我更新和分化成各种组织谱系(包括脂肪细胞,软骨细胞,肌细胞和成骨细胞谱系)的能力允许恢复损伤的组织。另外,推测ASCs有可能在体外复制组织。器官将允许更容易获得新颖药物的评估,从而显着降低药物生产成本。然而,隔离,维护和冷冻保存过程中的不一致,禁止集体分析世界各地不同实验室的结果。用于分离和培养ASC的标准方案对于确保一致的数据分析是必要的。
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Mouse Model of Dengue Virus Infection with Serotypes 1 and 2 Clinical Isolates
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Author:
Date:
2016-12-05
[Abstract] Dengue is a global public health threat caused by infection with any of the 4 related dengue virus serotypes (DENV1-4). Clinical manifestations range from self-limiting febrile illness, known as dengue fever (DF), to life-threatening severe diseases, such as dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Most cases of DHF/DSS are associated with secondary heterotypic infections through a phenomenon that is described as antibody-dependent enhancement of infection (ADE). There are an estimated 400 million human infections and several hundred thousand cases of severe dengue occurring yearly. At present, however, there are no approved antiviral drugs against DENV infection. The lack of a suitable animal model has hampered the evaluation of novel antiviral candidates for DENV ...
[摘要] 登革热是由4种相关登革热病毒血清型(DENV1-4)的任一种感染引起的全球公共卫生威胁。临床表现的范围从自限性发热疾病,称为登革热(DF),到危及生命的严重疾病,如登革热出血热(DHF)或登革热休克综合征(DSS)。大多数DHF/DSS病例通过描述为抗体依赖性增强感染(ADE)的现象与继发性异型感染相关。估计每年有4亿人感染和数十万例严重登革热病例。然而,目前,还没有批准的抗DENV感染的抗病毒药物。缺乏合适的动物模型阻碍了DENV感染的新抗病毒候选物的评价。由于DENV在免疫活性小鼠中不良地建立感染,已将AG129小鼠(缺乏I型和II型IFN [干扰素]受体)和小鼠适应的DENV2株应用于能够繁殖人类感染的几种主要病理的登革动物模型。最近,我们开发了具有临床分离株DENV1和DENV2的新的小鼠模型,其将用于药物测试和登革热发病机理研究(Watanabe等人,2016)。在这里我们描述建立临床分离株的登革热小鼠模型的细节;从体外材料制备到体内病毒感染。值得注意的是,由于DENV在小鼠中的感染性在病毒株之间不同,不是所有临床分离株都可以诱导严重的登革热。 关键字:登革热病毒,致命的小鼠模型,临床病毒,病毒感染的抗体依赖性增强,药物测试 [背景] 为了克服DENV在啮齿动物细胞中不能很好复制的缺点,多年来已经进行了许多努力来开发模拟人类登革热感染的小动物模型。近交小鼠模型系统允许实验可变性最小化,并且遗传工程小鼠模型能够再现动物中登革热临床症状的一些方面。过去的研究显示,用DENV2临床分离物感染的AG129小鼠(缺乏I型和II型IFN受体)感染瘫痪的迹象,这是中枢神经系统受累的病症,在人类病例中是罕见的(Shresta等人。,2004)。或者,产生可在AG129小鼠中诱导人类DHF/DSS样疾病的小鼠适应的DENV2毒株,并已用于登革热研究(Shresta等人,2006; ...
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