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Author:
Shih-Han Kao, Wen-Lung Wang, Chi-Yuan Chen, Yih-Leong Chang, Yi-Ying Wu, Yi-Ting Wang, Shu-Ping Wang, Alexey I Nesvizhskii, Yu-Ju Chen, Tse-Ming Hong and Pan-Chyr Yang,
Date:
2015-01-05
[Abstract] Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much popularity in cancer research. Here we present a full cycloheximide chase assay in our laboratory using a lung adenocarcinoma cell line, CL1-5, as a model.
[摘要] 真核细胞中蛋白质稳定性的比较已经通过放线菌酮实现，放线菌酮是蛋白质生物合成的抑制剂，由于其在翻译延伸中的预防。 它在确定给定蛋白质的半衰期方面广泛用于细胞生物学，并且已在癌症研究中获得广泛应用。 在这里，我们在我们的实验室使用肺腺癌细胞系CL1-5作为模型呈现完整的放线菌酮追踪测定法。
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