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Author:
Date:
2014-02-05
[Abstract] Biomineralization in vertebrates has both physiological and pathological aspects. Physiological mineralization is essential for proper development and function of hard tissues, such as bone, teeth, and growth plate cartilage, but it does not occur in soft tissues. Pathological ectopic mineralization, in contrast, occurs in soft tissues, including blood vessels, kidney, articular cartilage, and cardiovascular tissue. Here, we describe the simple method for detecting and measuring the presence of mineralized nodules in cardiac ventricular fibroblasts by using von Kossa and alizarin red S staining, and a colorimetric method for calcium quantification, respectively.
[摘要] 脊椎动物中的生物矿化具有生理和病理学方面。 生理矿化对于硬组织（例如骨，牙齿和生长板软骨）的正常发育和功能是必需的，但它不在软组织中发生。 相反，病理异位矿化发生在软组织中，包括血管，肾，关节软骨和心血管组织。 在这里，我们描述了使用von Kossa和茜素红S染色和钙定量的比色法分别检测和测量心室成纤维细胞中矿化结节的存在的简单方法。
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