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Author:
Date:
2013-11-05
[Abstract] Bromodeoxyuridine (BrdU) is a thymidine analog that is incorporated into DNA during the S-phase of the cell cycle. As such, BrdU incorporation can be used to quantify the number of cells that are in S-phase in the time period during which BrdU is available. The following protocol describes an in vivo BrdU incorporation assay as a measure of cell proliferation in adult murine hematopioetic stem cells (HSCs). Specifically, BrdU incorporation was analyzed for long-term HSCs (LT-HSCs, Lin-Sca-1+c-Kit+CD34-CD135-), Short-term HSCs (ST-HSCs, Lin-Sca-1+c-Kit+CD34+CD135-) and multipotent progenitors (MPPs, Lin-Sca-1+c-Kit+CD34+CD135+) population.
[摘要] 溴脱氧尿苷（BrdU）是在细胞周期的S期期间掺入DNA的胸苷类似物。 因此，BrdU掺入可用于定量在BrdU可用的时间段内处于S期的细胞的数量。 以下方案描述了体内BrdU掺入测定作为成体鼠造血干细胞（HSC）中细胞增殖的量度。 具体地，分析BrdU掺入的长期HSC（LT-HSC，Lin-Sca-1 + c-Kit + CD34 - CD135 - ），短期HSC（ST-HSC，Lin-Sca-1 + c-Kit + CD34 和多能祖细胞（MPP，Lin-Sca-1 + c-Kit + CD34 + > CD135 + ）群体
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