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Anti-MBP Antiserum

抗MBP抗血清

Company: New England Biolabs
Catalog#: E8030S
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In vitro Protein Ubiquitination Assays
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Date:
2013-10-05
[Abstract]  Ubiquitin can be added to substrate protein as a protein tag by the concerted actions of ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2) and ubiquitin protein ligase (E3). At the present of E1 and ubiquitin, E2 activity can be determined by the thio-ester formation. The E3 activity of a putative protein as well as the E2/E3 or E3/substrate specificities also can be explored by in vitro ubiquitination assay. The result can be detected by western blot with certain antibody. Purified proteins expressed from bacterial system are always used in this assay. [摘要]  泛素可以通过泛素激活酶(E1),泛素缀合酶(E2)和泛素蛋白连接酶(E3)的协同作用作为蛋白标签添加到底物蛋白中。 在E1和泛素的存在下,E2活性可以通过硫酯形成来确定。 推定的蛋白质的E3活性以及E2/E3或E3 /底物特异性也可以通过体外泛素化测定来研究。 结果可以通过用某些抗体的western印迹来检测。 在该测定中总是使用从细菌系统表达的纯化蛋白质。

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