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Author:
Date:
2013-06-05
[Abstract] DiOC2 (Novo et al., 2000) exhibits green fluorescence in all bacterial cells, but the fluorescence shifts towards red emission as the dye molecules self associate at the higher cytosolic concentrations caused by larger membrane potentials. Proton ionophores such as CCCP destroy membrane potential by eliminating the proton gradient. The magnitude of membrane potentials varies with different bacterial species. For many gram-positive species, including Staphylococcus aureus and Micrococcus luteus, the red:green ratio tends to vary with the intensity of the proton gradient while in many gram-negative bacteria such as Escherichia coli and Salmonella choleraesuis, the response of the dye does not appear to be proportional to proton ...
[摘要] DiOC 2（Novo等人，2000）在所有细菌细胞中表现出绿色荧光，但是随着染料分子在更高的胞质浓度自缔合，荧光向红色发射移动 由较大的膜电位引起。 质子离子载体如CCCP通过消除质子梯度来破坏膜电位。 膜电位的大小随不同的细菌种类而变化。 对于许多革兰氏阳性菌种，包括金黄色葡萄球菌和藤黄微球菌，红色：绿色比倾向于随着质子梯度的强度而变化，而在许多革兰氏阴性菌中 例如大肠杆菌和霍乱沙门氏菌，染料的响应似乎不与质子梯度强度成比例。 结核分枝杆菌本身是一种难以处理的生物体，因为其刚性细胞壁。
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