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Author:
Date:
2013-06-05
[Abstract] The primary aerial surfaces of all land plants are coated by a lipidic cuticle, which restricts non-stomatal water loss and protects the plant from pathogens, herbivores, and ultraviolet radiation. The cuticle is made up of two components: cutin, a polymer of hydroxy- and epoxy- long-chain fatty acid derivatives and glycerol, and cuticular waxes, which are derivatives of very-long-chain fatty acids. While chemical analysis of cutin can be a lengthy and technically challenging task, analysis of cuticular waxes is relatively simple, and can be routinely used to characterize different plant species, adaptations of a given species to environmental conditions, or mutant phenotypes. Here, we present a protocol tailored for the analysis of cuticular waxes on the surface of the model organism Arabidopsis ...
[摘要] 所有陆地植物的主要空间表面都涂有脂质角质层，限制非气孔水分流失，保护植物免受病原体，食草动物和紫外线辐射。角质层由两部分组成：角质，羟基和环氧长链脂肪酸衍生物的聚合物和甘油，以及作为非常长链脂肪酸衍生物的角质蜡。虽然角质层的化学分析可能是一个漫长而技术上具有挑战性的任务，但是角质层蜡的分析相对简单，可以常规用于表征不同植物物种，给定物种适应环境条件或突变体表型。在这里，我们提出了一种针对拟南芥模拟生物表面上的角质层蜡分析的方案。因为在植物的最外表面上发现角质蜡，蜡提取过程非常简单，样品处理可在不到一天的时间内完成。化学分析包括通过气相色谱与火焰离子化检测（GC / FID）联用对蜡单体进行定量，以及通过质谱法或单个蜡组分与已知标准物的保留时间的比较来鉴定蜡单体。
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