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Author:
Date:
2021-02-05
[Abstract] Lipids metabolism is comprised of networks of reactions occurred in different subcellular compartments. Isotopic labeling is a good way to track the transformations and movements of metabolites without perturbing overall cellular metabolism. Fatty acids, the building blocks of membrane lipids and storage triacylglycerols, are synthesized in plastids. The immediate precursor for fatty acid synthesis is acetyl-CoA. Exogenous acetate is rapidly incorporated into fatty acids in leaves and isolated plastids because it can diffuse freely through cellular membranes, enter the plastid where it is rapidly metabolized to acetyl-CoA. Therefore, isotope-labeled acetate is often used as a tracer for the investigation of fatty acid synthesis and complex lipid metabolism in plants and other organisms. ...
[摘要] [摘要]脂质代谢由发生在不同亚细胞区室的反应网络组成。同位素标记是跟踪代谢物的转化和运动的好方法，而不会干扰整个细胞的新陈代谢。发TTY酸，膜脂和存储的构建块的三酰基甘油，在质体中合成的。脂肪酸合成的直接前体是乙酰辅酶A。外源乙酸盐可快速掺入叶片和分离的质体中的脂肪酸中，因为它可以通过细胞膜自由扩散，进入质体，然后迅速代谢成乙酰辅酶A。 因此，同位素标记的乙酸盐通常用作研究植物和其他生物中脂肪酸合成和复杂脂质代谢的示踪剂。同位素标记的基本原理及其最新技术进展已得到综述（Allen等，2015）。本协议描述了使用 的14 C标记的乙酸，以确定的脂肪酸合成和降解速率和跟踪的代谢甘油脂中的叶子。该方法通常被称为醋酸酯脉冲追踪标记法，已被广泛用于探查脂质代谢的各个方面（Allen等，2015），包括自噬在膜脂质更新中的作用（Fan等，2015）。，2019）和脂质与淀粉代谢途径之间的相互作用（Yu et al。，2018）。
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Author:
Date:
2013-06-05
[Abstract] The primary aerial surfaces of all land plants are coated by a lipidic cuticle, which restricts non-stomatal water loss and protects the plant from pathogens, herbivores, and ultraviolet radiation. The cuticle is made up of two components: cutin, a polymer of hydroxy- and epoxy- long-chain fatty acid derivatives and glycerol, and cuticular waxes, which are derivatives of very-long-chain fatty acids. While chemical analysis of cutin can be a lengthy and technically challenging task, analysis of cuticular waxes is relatively simple, and can be routinely used to characterize different plant species, adaptations of a given species to environmental conditions, or mutant phenotypes. Here, we present a protocol tailored for the analysis of cuticular waxes on the surface of the model organism Arabidopsis ...
[摘要] 所有陆地植物的主要空间表面都涂有脂质角质层，限制非气孔水分流失，保护植物免受病原体，食草动物和紫外线辐射。角质层由两部分组成：角质，羟基和环氧长链脂肪酸衍生物的聚合物和甘油，以及作为非常长链脂肪酸衍生物的角质蜡。虽然角质层的化学分析可能是一个漫长而技术上具有挑战性的任务，但是角质层蜡的分析相对简单，可以常规用于表征不同植物物种，给定物种适应环境条件或突变体表型。在这里，我们提出了一种针对拟南芥模拟生物表面上的角质层蜡分析的方案。因为在植物的最外表面上发现角质蜡，蜡提取过程非常简单，样品处理可在不到一天的时间内完成。化学分析包括通过气相色谱与火焰离子化检测（GC / FID）联用对蜡单体进行定量，以及通过质谱法或单个蜡组分与已知标准物的保留时间的比较来鉴定蜡单体。
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