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Author:
Date:
2013-05-05
[Abstract] This method allows you to isolate different subclass mononuclear cells, like B-cells, T cells, CD4+ and CD8+ T, from mouse spleen. By conjugating cells with specific antibodies and subsequently magnetic beads isolation, using the technique from Miltenyi, this allows a high purity.
[摘要] 这种方法允许你从小鼠脾脏中分离不同的亚类单核细胞，如B细胞，T细胞，CD4 +和CD8 + T。 通过使用来自Miltenyi的技术使细胞与特异性抗体缀合并随后磁珠分离，这允许高纯度。
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