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illustra GFX PCR DNA and Gel Band Purification Kits

illustra GFX PCR DNA和凝胶带纯化试剂盒

Company: GE Healthcare
Catalog#: 28-9034-71
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Cellular Translational Reporter Assay
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Date:
2014-03-20
[Abstract]  The method described here allows measuring the effect of exogenously introduced modifications to in vitro-transcribed mRNA on the translation in cells. Using cells derived from knockout mice and control littermates, this method enables to compare the results in the presence or absence of specific gene products. In our lab, we used this protocol to check whether the exogenous addition of 5’ capping and 2’-O methylation to in vitro-mRNA affects the translational efficiency. Here we describe the details of our experiments. [摘要]  本文所述的方法允许测量外部引入的修饰对体外转录的mRNA对细胞翻译的影响。 使用来自敲除小鼠和对照同窝出生的细胞,该方法能够比较存在或不存在特定基因产物的结果。 在我们的实验室中,我们使用该协议来检查外部添加5'帽化和2'-O甲基化在体外 -mRNA是否影响翻译效率。 这里我们描述我们的实验的细节。

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